![]() Proceedings of the National Academy of Sciences of USA, 106, 1099310998. (2009) Derivation of Induced Pluripotent Stem Cells from Pig Somatic Cells. Įzashi, T., Telugu, V.P.V.L., Alexenko, A.P., Sachdev, S., Sinha, S. Journal of Molecular Cell Biology, 1, 46-54. (2009) Generation of Pig Induced Pluripotent Stem Cells with a Drug-Inducible System. The Journal of Biological Chemistry, 284, 17634-17640. (2009) Generation of Induced Pluripotent Stem Cell Lines from Tibetan Miniature pig. (2008) Porcine Embryonic Stem Cells: A Possible Source for Cell Replacement Therapy. Journal of Reproduction Fertility-Supplement, 41, 51-56. (1990) Maintenance and Differentiation in Culture of Pluripotential Embryonic Cell Lines from Pig Blastocysts. (1990) A Method for Cultivating Morphologically Undifferentiated Embryonic Stem Cells from Porcine Blastocysts. (2012) Concise Review: Induced Pluripotent Stem Cells Versus Embryonic Stem Cells: Close Enough or Yet Too Far Apart? Stem Cells, 30, 33-41. (2006) Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors. (1958) Sexually Mature Individuals of Xenopus laevis from the Transplantation of Single Somatic Nuclei. (2009) Naive and Primed Pluripotent States. Summarize the major signaling pathways involved in maintenance of pluripotencyĪnd the state of the art conceptual and technical progress for generating bona Investigations and applications of porcine stem cells. Generate stem cells has dramatically revolutionized the basic and applied Genetic engineering, innovative cell culturing and induced pluripotency to Advances in stem cell technology, especially the Stem cells are under increasing glare of publicity due to milestone achievements More than two decades with slow progress. Attempts to derivepluripotent stem cells (PSCs) from porcine have been going on for This could open a newĪvenue of research into degenerative conditions as porcine is a more effectiveįide PSCs are currently available onlyin rodents (mouse, rat) and primates Important clinical insights for cardiac cell therapy. Porcines ( Sus scrofa ) are excellent models for cardiovascular diseaseĪnd pluripotent stem cells (PSCs) generated from porcines will provide Pathway meaning that research has to be focused on cell transplantation. Unlike lower vertebrate species the human heart lacks a regenerative ![]() The chemically fixed autologous feeders may be used as a substitute for large scale culture of hiPS cells as a convenient in-house and a cost-effective method.Human heart following regenerative diseases remain a challenge for medical Furthermore, hiPS cells cultured on chemically fixed feeders formed teratomas in vivo, characterized by all three germ layers. Treatment of (2.5% glutaraldehyde or formaldehyde for 10 min) autologous human dermal fibroblasts allowed hiPS cells to adhere and grow as undifferentiated colonies characterized by the expression of pluripotency markers such as alkaline phosphatase, Oct-3/4, and stage-specific embryonic antigen-4. Here, we employed chemically fixed autologous feeder cells for hiPS cell culture without additional time needed for colony formation. Furthermore, recombinant systems require a long time for colony formation. Alternatively, feeder-free culture of hiPS cells requires high cell seeding densities, specialized culture medium, and growth supplements, which raise the cost. Conventional culture of human induced pluripotent (hiPS) cells requires feeder cell preparation that is time consuming and labour intensive.
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